Fig. 2.
Glucocorticoid Regulation of 11β-HSD1 in A549 Cells Is Indirect and Dependent upon New Protein Synthesis
Real-time PCR measurement of 11β-HSD1 mRNA levels, expressed as fold induction, relative to levels in vehicle-treated cells. A, 11β-HSD1 mRNA levels were increased within 4 h of addition of 10−6 m dexamethasone to A549 cells and were maximal at 24 h. B, Dexamethasone (Dex, 24 h) increased endogenous 11β-HSD1 mRNA levels in a dose-dependent manner in A549 cells. C, The GR antagonist RU38486 (RU486, 10−5 m), added 30 min before 10−6 m dexamethasone, blocked the induction of 11β-HSD1 mRNA. D, Dexamethasone induction of 11β-HSD1 mRNA in A549 cells was blocked by cycloheximide (CHX, 10−5 m) added 30 min before 10−6 m dexamethasone (4 h). Data are mean ± sem of at least three independent mRNA samples. ***, P < 0.0001.