Fig. 7.
C/EBPβ Occupancy of the 11β-HSD1 Promoter Is Increased after Dexamethasone
Representative ChIP assay showing association of C/EBPβ with the endogenous 11β-HSD1 P2 promoter in A549 cells after 24 h treatment with 10−6 m dexamethasone. PCR (35 cycles) were carried out with primers spanning FP3 and FP4 on DNA after immunoprecipitation with control IgG (lanes 7 and 8), C/EBPβ antiserum (lanes 9 and 10), RNA polymerase II antiserum (RNAP; lanes 11 and 12) or after control precipitations with no antibody (No Ab, lanes 5 and 6). Lane 1 contains marker, and lane 2 a PCR carried out with water as negative control. Lanes 3 and 4 show input DNA (1%) from vehicle-treated or dexamethasone-treated cells, respectively. The assay shown is representative of two independent assays.