Fig. 8.
Overexpression of C/EBPβ Blocks the Effect of Glucocorticoid on the 11β-HSD1 P2 Promoter
Ectopic expression of C/EBPβ increased basal promoter activity and attenuated the dexamethasone response of the 11β-HSD1 P2 promoter in transiently transfected A549 cells. Ectopic expression of C/EBPδ had no effect. In each transfection, 1 × 105 cells were seeded per well in six-well plates and transfected with 250 ng 11β1(−196/+49), 250 ng pRSV-lacZ (internal control), and 50 ng C/EBP expression plasmid or empty vector. Promoter activity is expressed as luciferase/β-galactosidase (internal control) activity. Data are expressed relative to vehicle-treated 11β1(−196/+49) cotransfected with empty vector (vector; arbitrarily set to 1) and are mean ± sem of at least three experiments, each carried out in triplicate. *, Significantly different from vehicle-treated control, P < 0.05; ***, P < 0.0001. §, Significantly different from vector, P < 0.05.