Fig. 2.
E2 Down-Regulates E-Cadherin Expression
A, BG-1 cells were treated with vehicle (Cont) or increasing concentrations of E2 (10−15 to 10−7 m) for 48 h. B, BG-1 cells were treated with either vehicle (Cont), 10−7 m E2 or in combination with 10−7 m ICI for 24, 48, and 72 h. Whole-cell lysates was extracted, and E-cadherin protein (E-Cad) was detected by Western blot. β-Actin was used as a loading control. The immunoblot shown is representative of three independent experiments. Cumulative results for quantitative densitometry of the three experiments are shown in lower panels. Mean ± sd values are depicted for protein abundance expressed as percentage in control cells. *, P < 0.05 compared with control. +, P < 0.05 compared with the corresponding time-matched vehicle control. Cont, Control.