Fig. 7.
ERβ Up-Regulates E-Cadherin and Inhibits Cell Migration
A, SKOV-3 cells were transfected with nonspecific siRNA (NS siRNA), ERα siRNA or ERβ siRNA. Efficiencies of the siRNA were confirmed by Western blot with ERβ antibody. B, SKOV-3 cells were transfected with the siRNAs and subsequently treated with 10−7 m E2 for 48 h. E-cadherin (E-Cad) and β-actin were detected by Western blot. C, Scratch was made by scraping the monolayer 24 h after siRNA transfection, and cells were incubated with medium containing vehicle (Cont) or 10−7 m E2. Wound closure was photographed after 24 h. The amount of wound repair was expressed as uncovered area compared with initial uncovered area of vehicle-treated control at time zero. *, P < 0.05 compared with control. Numerical values below each lane of the immunoblots represent quantification of the relative protein level by densitometry (normalized to β-actin protein level). Cont, Control.