Fig. 2.

Knockdown of NUR77 Reduces the cAMP-Mediated Induction of StAR Expression

MA-10 Leydig cells were transfected with siRNA directed against Nur77 and then treated with FSK (10 μm) for the indicated time. Total RNA were isolated, and expression of Nur77 (A) and StAR (B) was determined by quantitative real-time PCR. Results were corrected with the Rpl19 cDNA. Results are the mean of three individual experiments performed in duplicate (±sem). An asterisk indicates a statistically significant difference. In C, Western blots were performed to assess the efficiency of Nur77 siRNA knockdown at the protein level (middle panel) and the impact on StAR protein level (top panel). Tubulin was used as control (lower panel).