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. 2017 Feb 3;206(1):135–150. doi: 10.1534/genetics.116.198911

Figure 3.

Figure 3

Identification of the Tam3Ld insertion marking the proximal border of the Wc macro-transposon. (a) PCR amplification of the Tam3Ld-3′ junction from Wc plants. Candidate Tam3L-3′ junction sequences were amplified by TAIL-PCR anchored by locus-specific primers in the predicted location of Tam3Ld (see Materials and Methods). The TAIL-PCR sequences were used to design a pair of sequence-specific primers, Wc3P-R3 and Wc3P-F3, that amplified a 794-bp junction fragment (agarose gel electrophoresis image). The locations of sequence-specific primers are diagramed on the right. Green indicates transposon sequence. The 8-bp target site duplication is gray. (b) Confirmation of the Wc macro-transposon insertion site. The Tam3Ld junction sequence aligned to the B73 reference genome at a position that was contiguous with the sequence flanking Tam3La-5′ and included a matching 8-bp target site duplication (gray). The Tam3Ld and Tam3La 12-base inverted terminal repeats are colored green. B73 chromosome 9 genome coordinates (version 3) are indicated below the sequence.