Figure 4.

Structural heterogeneity in Wc repeats. (a) Ccd1 and Tglu copy number vary uniformly in Wc alleles. Ccd1 and Tglu copy numbers were estimated using k-mer frequency analysis. The line was determined by linear regression with the equation and R² value indicated. (b) Variation in P450 copy number relative to Ccd1 reveals structural heterogeneity in Wc repeats. Gene copy number was estimated as described in Materials and Methods. Two groups of Wc alleles that differ in P450:Ccd1 copy number ratio are indicated by the dashed ovals. Open symbols, alleles having comparatively high P450:Ccd1 ratios.