Figure 2.

Marcal1 mutants are sensitive to killing by DSB-inducing agents. Flies heterozygous for null mutations in Marcal1 were mated in two broods of at least 10 vials, with each vial representing a biological replicate. Brood one was unexposed; brood two received a dose of MMS, HN2, HU, ETS, CPT, or IR during larval feeding. Relative survival was calculated as the ratio of homozygous mutant to heterozygous control adults in treated vials, normalized to the same ratio in the corresponding unexposed vial. Dotted line represents 100% relative survival. Dosage, number of replicates, and total progeny counts are in Table S1 in File S1. **** P < 0.0001 in paired t-tests between unexposed and exposed vials.