Figure 7.

Hermes excision frequencies of different mre11 mutants. Each data point represents the excision frequency from a WT or mutant cell culture analyzed by PCR (Figure 6). The red bar shows the median frequency. The differences between mre11⁺ WT and mre11-L77K and mre11-L77KL174D were statistically significant (P < 0.001 and 0.002, respectively, by Mann–Whitney two-tailed rank sum test), the difference between mre11⁺ WT and mre11-H68S was borderline for significance in this nonparametric test (P = 0.023), while the differences between mre11⁺ and mre11-H134S or ctp1∆ mre11-H134S, and between mre11-H134S and ctp1∆ mre11-H134S, were not distinguishable (P = 0.218, 0.684, and 0.143, respectively). NHEJ, nonhomologous end-joining; wild-type, WT.