Table 1. Yield of transposon insertions from the different transient transfection permutations in Figure 1.
| Medium | Transforming DNA (μg) | G418R FOAR Transformantsa | |
|---|---|---|---|
| Total | #/μg | ||
| YES | 0.3 | 20 | 67 |
| 1.0 | 66 | 66 | |
| 3.0 | 95 | 32 | |
| EMM + aluh | 0.3 | 325 | 1083 |
| 1.0 | 690 | 690 | |
| 3.0 | 804 | 268 | |
FOA, 5’-fluoroorotic acid; YES, yeast extract sucrose; aluh, adenine, leucine, uracil, and histidine.
a
Number of colonies on the final YES + G418 + FOA plates following growth on nonselective medium in which transposase is (EMM) or is not (YES) induced.