Fig. 7.

Ternary Interactions between SRC-1, NCoR, and PPAR

A, SRC-1 overexpression. HeLa cells were cotransfected with either full-length WT or V227A PPARα (50 ng), a reporter construct driven by residues −1081 to +22 of the HMGCS2 promoter (100 ng) and increasing amounts of full-length SRC-1 (50 and 100 ng) with 100 μm WY 14,643. Values (mean ± se of three replicates) are expressed as percentage of maximal WT activity. B, Effects of SRC-1 on NCoR/PPARα interactions. HeLa cells were cotransfected with chimeric VP16-WT, VP16-V227A (100 ng), and Gal-NCoR (100 ng) together with the (UASG)₅-Luc reporter (500 ng). Cells were exposed to WY14,643 (10 μm) at increasing doses (50, 100, and 200 ng) of full-length SRC-1. Fold interaction (mean ± sd of three replicates) was expressed as fold of VP16 empty vector. *, P < 0.05; **, P < 0.01.