Fig. 3.

The Stabilization of N-CoR/SMRT Corepressor Complexes by LisH Domain

A, Flag-tagged TBL1, TBLR1, TBL1ΔLisH, and TBLR1ΔLisH constructs were transfected into HeLa cells. Immunoprecipitation was performed (left), and the precipitated materials were analyzed by Western blot with antibodies to the specific proteins listed on the right. B, HeLa nuclear extracts transfected with the indicated plasmids (Flag-tagged TBLR1 and TBLR1ΔLisH) were separated by Superose 6 gel filtration, and fractions were analyzed by Western blot with antibodies to the specific proteins indicated on the right. IP, Immunoprecipitation; WB, Western blot.