Fig. 7.

Regulation of Endogenous GR Target Genes by Phosphorylation/Sumoylation

A549 cells were transfected with pcDNA3 (indicated by the solid line in panels A and B and lanes 1 and 2 in panel C), PEBG-JNK1α1 (1 μg), and pcDNA3HA-MLK3 (0.5 μg), (indicated by the dashed line in panel A and lanes 3, 4, 7, and 8 in panel C) and SUMO2 (1 μg, indicated by the dotted line in panel B, and lanes 5–8 in panel C). Cells were treated with 100 nm Dex for the times indicated (1 h for 7C), RNA was isolated, and reverse transcription was performed. Obtained cDNA was used for qRT-PCR reactions as described in Materials and Methods. Graphs represent the average of three or more separate experiments.