Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1976 Jan;57(1):15–22. doi: 10.1104/pp.57.1.15

Incorporation of Cytokinin N6-Benzyladenine into Tobacco Callus Transfer Ribonucleic Acid and Ribosomal Ribonucleic Acid Preparations 1

Donald J Armstrong a,2, Norimoto Murai a, Barbara J Taller a, Folke Skoog a
PMCID: PMC541955  PMID: 16659417

Abstract

The incorporation of the cytokinin N6-benzyladenine into tobacco (Nicotiana tabacum) callus tRNA and rRNA preparations isolated from tissue grown on medium containing either N6-benzyladenine-8-14C or N6-benzyladenine-8-14C: benzene-3H(G) has been examined. N6-benzyladenine was incorporated into both the tRNA and rRNA preparations as the intact base. Over 90% of the radioactive N6-benzyladenosine recovered from the RNA preparations was associated with the rRNA. Purification of the crude rRNA by either MAK chromatography or Sephadex G-200 gel filtration had no effect on the N6-benzyladenosine content of the RNA preparation. The distribution of N6-benzyladenosine moieties in tobacco callus tRNA fractionated by BD-cellulose chromatography did not correspond to the distribution of ribosylzeatin activity. N6-benzyladenosine was released from the rRNA preparation by treatment with venom phosphodiesterase and phosphatase, ribonuclease T2 and phosphatase, or ribonuclease T2 and a 3′-nucleotidase. N6-benzyladenosine was not released from the RNA preparation by treatment with either ribonuclease T2 or phosphatase alone or by successive treatment with ribonuclease T2 and a 5′-nucleotidase. Brief treatment of the rRNA preparation with ribonuclease T1 and pancreatic ribonuclease converted the N6-benzyladenosine moieties into an ethyl alcohol soluble form. On the basis of these and earlier results, the N6-benzyladenosine recovered from the tobacco callus RNA preparations appears to be present as a constituent of RNA and not as a nonpolynucleotide contaminant.

Full text

PDF
20

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armstrong D. J., Burrows W. J., Evans P. K., Skoog F. Isolation of cytokinins from tRNA. Biochem Biophys Res Commun. 1969 Oct 22;37(3):451–456. doi: 10.1016/0006-291x(69)90936-x. [DOI] [PubMed] [Google Scholar]
  2. Armstrong D. J., Skoog F., Kirkegaard L. H., Hampel A. E., Bock R. M., Gillam I., Tener G. M. Cytokinins: distribution in species of yeast transfer RNA. Proc Natl Acad Sci U S A. 1969 Jun;63(2):504–511. doi: 10.1073/pnas.63.2.504. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bartz J. K., Söll D. N 6 -( 2 -isopentenyl) adenosine: biosynthesis in vitro in transfer RNA by an enzyme purified from Escherichia coli. Biochimie. 1972;54(1):31–39. doi: 10.1016/s0300-9084(72)80035-x. [DOI] [PubMed] [Google Scholar]
  4. Burrows W. J., Skoog F., Leonard N. J. Isolation and identification of cytokinins located in the transfer ribonucleic acid of tobacco callus grown in the presence of 6-benzylaminopurine. Biochemistry. 1971 Jun 8;10(12):2189–2194. doi: 10.1021/bi00788a002. [DOI] [PubMed] [Google Scholar]
  5. Fox J. E., Chen C. Characterization of labeled ribonucleic acid from tissue grown on 14C-containing cytokinins. J Biol Chem. 1967 Oct 10;242(19):4490–4494. [PubMed] [Google Scholar]
  6. Fox J. E. Incorporation of a kinin, N, 6-benzyladenine into soluble RNA. Plant Physiol. 1966 Jan;41(1):75–82. doi: 10.1104/pp.41.1.75. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gillam I., Millward S., Blew D., von Tigerstrom M., Wimmer E., Tener G. M. The separation of soluble ribonucleic acids on benzoylated diethylaminoethylcellulose. Biochemistry. 1967 Oct;6(10):3043–3056. doi: 10.1021/bi00862a011. [DOI] [PubMed] [Google Scholar]
  8. HALL R. H. ISOLATION OF N6-(AMINOACYL)ADENOSINE FROM YEAST RIBONUCLEIC ACID. Biochemistry. 1964 Jun;3:769–773. doi: 10.1021/bi00894a006. [DOI] [PubMed] [Google Scholar]
  9. Hall R. H. N6-(delta 2-isopentenyl)adenosine: chemical reactions, biosynthesis, metabolism, and significance to the structure and function of tRNA. Prog Nucleic Acid Res Mol Biol. 1970;10:57–86. doi: 10.1016/s0079-6603(08)60561-9. [DOI] [PubMed] [Google Scholar]
  10. Kline L. K., Fittler F., Hall R. H. N6-(delta-2-isopentenyl) adenosine. Biosynthesis in transfer ribonucleic acid in vitro. Biochemistry. 1969 Nov;8(11):4361–4371. doi: 10.1021/bi00839a021. [DOI] [PubMed] [Google Scholar]
  11. Laloue M., Terrine C., Gawer M. Cytokinins: formation of the nucleoside-5'-triphosphate in tobacco and Acer cells. FEBS Lett. 1974 Sep 15;46(1):45–50. doi: 10.1016/0014-5793(74)80331-5. [DOI] [PubMed] [Google Scholar]
  12. Loening U. E. The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis. Biochem J. 1967 Jan;102(1):251–257. doi: 10.1042/bj1020251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. MANDELL J. D., HERSHEY A. D. A fractionating column for analysis of nucleic acids. Anal Biochem. 1960 Jun;1:66–77. doi: 10.1016/0003-2697(60)90020-8. [DOI] [PubMed] [Google Scholar]
  14. Murai N., Armstrong D. J., Skoog F. Incorporation of mevalonic Acid into ribosylzeatin in tobacco callus ribonucleic Acid preparations. Plant Physiol. 1975 May;55(5):853–858. doi: 10.1104/pp.55.5.853. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Nishimura S. Minor components in transfer RNA: their characterization, location, and function. Prog Nucleic Acid Res Mol Biol. 1972;12:49–85. [PubMed] [Google Scholar]
  16. Playtis A. J., Leonard N. J. The synthesis of ribosyl-cis-zeatin and thin layer chromatographic separation of the cis and trans isomers of ribosylzeatin. Biochem Biophys Res Commun. 1971 Oct 1;45(1):1–5. doi: 10.1016/0006-291x(71)90041-6. [DOI] [PubMed] [Google Scholar]
  17. Rogozinska J. H., Helgeson J. P., Skoog F. Partial Purification of a Cell-Division Factor from Peas. Plant Physiol. 1965 May;40(3):469–476. doi: 10.1104/pp.40.3.469. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rosenbaum N., Gefter M. L. Delta 2 -isopentenylpyrophosphate: transfer ribonucleic acid 2 -isopentenyltransferase from Escherichia coli. Purification and properties of the enzyme. J Biol Chem. 1972 Sep 25;247(18):5675–5680. [PubMed] [Google Scholar]
  19. Söll D. Enzymatic modification of transfer RNA. Science. 1971 Jul 23;173(3994):293–299. doi: 10.1126/science.173.3994.293. [DOI] [PubMed] [Google Scholar]
  20. Tzou D. S., Galson E. C., Sondheimer E. The Metabolism of Hormones during Seed Germination and Release from Dormancy: III. The Effects and Metabolism of Zeatin in Dormant and Nondormant Ash Embryos. Plant Physiol. 1973 May;51(5):894–897. doi: 10.1104/pp.51.5.894. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Walker G. C., Leonard N. J. The mode of incorporation of 6-benzylaminopurine into tobacco callus transfer ribonucleic Acid: a double labeling determination. Plant Physiol. 1974 Nov;54(5):737–743. doi: 10.1104/pp.54.5.737. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Zachau H. G. Transfer ribonucleic acids. Angew Chem Int Ed Engl. 1969 Oct;8(10):711–727. doi: 10.1002/anie.196907111. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES