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Aldosterone Induces Activation of nPKC Isoforms
M1-CCD cells were treated with aldosterone (10 nm), and autophosphorylation of nPKC isoforms was determined by Western blotting over a time course of 30 min. Autophosphorylation of PKCδ was determined using a phospho-Ser643-specific antibody (A), and autophosphorylation of PKCε was determined using a phospho-Ser729-specific antibody (B). Values represent the means of six separate experiments. ***, P < 0.001 vs. control.
