Fig. 7.

SGK-1 Induction by Aldosterone Is Not Sensitive to PKD1 Suppression

Lysates were prepared from wild-type M1-CCD cells (PKD+) and M1-CCD cells suppressed in PKD1 expression (PKD−) either treated with aldosterone (10 nm) for 30 min (A), vehicle treated for 30 min (V), or not treated (C). Equivalent amounts were separated on SDS-PAGE gel (80 μg protein) and then Western blotted and probed using a SGK-1-specific antibody. Values represent the means of four separate experiments. **, P < 0.01. Expression of β-actin was used as a control for gel loading.