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. 2017 May 5;12(5):e0177089. doi: 10.1371/journal.pone.0177089

Fig 4. M2 macrophage number increases after co-culture with MDA-MB-231-SUSD2.

Fig 4

Macrophages co-cultured with MDA-MB-231-SUSD2 and MDA-MB-231-vector are shown. SC monocyte cell line was differentiated with PMA to generate SC M0 macrophages. MDA-MB-231-SUSD2 or vector cells were then added and co-cultured with SC M0 macrophages for 24 hours. A) Cells were collected, pelleted, fixed, paraffin-embedded and sectioned for IHC staining of CD68 and CD163. Cells were counterstained with hematoxylin. Images were taken at 200x. B) M2 macrophage percentage was determined by dividing CD163 (M2 macrophage marker) positive cells by CD68 (pan macrophage marker) positive cells in serial sections for three fields per condition. ImageJ software was used to count positively stained cells. Quantification is from a representative experiment of two biological replicates.