Fig. 2.

TGFβ1 Inhibits mRNA and Protein Expression of PR and Increases Protein Expression of PIASγ

A, Time course of PR mRNA expression in cultured ESCs decidualized in vitro with or without TGFβ1 measured by Q-RT-PCR. TGFβ1 reduces expression of PR mRNA in a time-dependent manner in primary ESCs. Results are ± sem (P < 0.05); n = 6 endometrial samples. B, Immunohistochemical analysis of PR expression in control and TGFβ1-treated ESC cells; PR immunostaining of ESCs decidualized in vitro (I) and ESCs decidualized in vitro plus TGFβ1 (10 ng/ml) (II). Inset shows negative control (matched isotype control). C, Whole-cell lysates from primary ESC cultures decidualized in vitro for 24 or 72 h, with or without TGFβ1 (10 ng/ml), were subjected to Western blot analysis. TGFβ1 treatment reduced protein expression of both PR-A and PR-B in decidualized ESCs and increased protein expression of PIASγ in decidualized ESC. *, P < 0.05; ***, P < 0.001.