Fig. 5.

Anti-TGFβ1 Antibody Neutralizes Endogenous TGFβ1 and Potentiates PR Expression but Has No Effect on DKK Protein Release

Cultured ESCs were decidualized in vitro with or without TGFβ1 treatment. To confirm the specificity of the TGFβ1 response, anti-TGFβ1 (1 μg/ml) or mouse IgG control was added, for 72 h. A, TGFβ1 reduces expression of mRNA nuclear PR, whereas anti-TGFβ1 antibody increases nuclear PR. B, TGFβ1 reduces protein release of DKK, whereas anti-TGFβ1 antibody has no effect. Results are ± sem (P < 0.05); n = 5 endometrial samples. ***, P < 0.001.