Fig. 10.

Effect of Concanavalin, DN β-Arrestin, and CHC siRNA on UCN-II-Induced ERK1/2 and p38MAPK Activation in 293-R2β Cells

Cells were pretreated with or without concanavalin A (0.25 mg/ml for 40 min) or alternatively transfected with 5 μg of either empty pcDNA3 vector (control) or β-arrestin (319–418) or 1 nmol CHC siRNA or scrambled oligonucleotide,and the effect on UCN-II (100 nm for 5 in) stimulation on ERK1/2 and p38MAPK activation was determined, by measurement of ERK1/2 and p38MAPK phosphorylation as described in Materials and Methods. Data represent the mean ± sem of two estimations from three independent experiments. *, P < 0.05 compared with control cells.