Table 3.

Primers and Annealing Temperatures Used

A.
NEO1an	5′-GTTTGCAGAATCCCTGCTTC-3′
Genom3	5′-CATGGTTCTAGAGGGCCACA-3′
ex4s	5′-GGCACGGTTCCACTTCAAAT-3′
ex4a	5′-CGGGTTTGATGGCACAACTT-3′
NEOs1	5′-GACCCATGGCGATGCCTGCTTG-3′
B.
P1	5′-TCCGCAGGAATGCAATTGTTGCTG-3′
P2	5′-GGGAAAGCATTACAAGTCTGGTTC-3′	Tm 60
E1	5′-TTGCTAGAGTGGAGCTTGCC-3′
E2	5′-GCCTAAATGGTGGAGGCATTC-3′	Tm 60
β-A	5′-TTGGCCTTAGGGTTCAGGGGG-3
β-A	5′-CGTGGGCCGCCCTAGGCACCA-3′	Tm 60

Table 3A lists PCR primers used in ES cell screening and mouse genotyping; Table 3B lists semiquantitative RT-PCR primers. Tm, Melting temperature.