Fig. 4.

Evolutionary Conservation of ERα-Binding Regions

A, Mean mouse-human conservation of ERα-binding regions using whole-genome alignments. The histogram shows the mean conservation index for all ERα-binding regions. A value of 0 means no conservation or that the regions cannot be aligned. B, The same analysis as in panel A but using randomly selected genomic regions of similar length as input. C, Conservation profile based on mean PhastCons scores (conservation between four vertebrates) of every nucleotide in ERα-binding regions, centered on the midpoint of each ERα-binding region. Black and gray lines denote ERα-binding regions and randomly selected genomic regions, respectively.