Fig. 3.

Inhibition of CDKs Blocks Phosphorylation of SF1 in Adrenocortical Cells

A, H295R and Y1 cells were incubated with DMSO (−), increasing concentrations of roscovitine (Rosc; 3, 10, or 30 μm) or U0186 (U0; 10 or 20 μm) for 1 h. SF1 was isolated by DPD, and Western blot analyses were performed using the anti-P-S-sub-CDKs antibody followed by the SF1 antibody. B, H295R and Y1 cells were incubated with DMSO (−), U0186 (U0; 10 μm), or roscovitine (Rosc; 30 μm) for 45 min. Cell extracts were run on SDS-PAGE and blotted with the phospho-ERK1/2 antibody (upper panel) followed by the ERK1/2 antibody (lower panel).