Fig. 6.

Mutated CDK7 Inhibits SF1-Dependent Transactivation

COS-1 cells were transfected with CYP17/Luc (1 μg) together with expression plasmids encoding SF1 and a mutated form of CDK7 (CDK7-K41R) (mCDK7; 50–250 ng) (panel A) or CDK2 (mCDK2; 50–250 ng) (panel B). The luciferase activities are given as fold induction relative to the luciferase activities in cells not transfected with Flag-pCMV2/SF1. Luciferase activities were calculated relative to β-gal activity (from pCMV5/βgal; 100 ng). The experiments were repeated at least three times (n ≥11), shown as means ± sds. *, P < 0.05; **, P < 0.01, as determined by one-way ANOVA Bonferoni posttest. The effect of mCDK7 on the pCMV5/βGAL promoter activity is shown in A, right panel.