Fig. 7.

Mutation in XPD of TFIIH Decreases SF1-Dependent Transcription and Phosphorylation

A, Hela and HD2 cells were transfected with SFRE/Luc, StAR/Luc, or MC2R/Luc (0.5 μg) and pCMV5/LacZ, in the absence or presence of Flag-pCMV2/SF1 (50 ng). The luciferase values are shown as fold increase in the presence of SF1 and relative to the β-gal activity (left panels). The luciferase and the β-gal activities are also shown separately in the middle and right panels, respectively. The experiments were repeated two or three times, shown as means ± sds (SFRE/Luc, n = 5; StAR/Luc, n =12; MC2R/Luc, n = 6). *, P < 0.05; **, P < 0.01; ***, P < 0.001; as determined by unpaired t test (folds) or by ANOVA, Bonferonni (luciferase and β-gal activities). B, Lysates from panel A were incubated with 0.1% SDS and subjected to Western blot analyses with a SF1 antibody to verify equal SF1 expression. C, HeLa and HD2 cells were transfected with Flag-pCMV2/SF1. SF1 was isolated by DPD and Western blotted with anti-P-Sub-CDK (upper panel) and SF1 (lower panel) antibodies. ACTHR, ACTH receptor.