Binding and Adenylyl Cyclase Activity Assays of Bpa28-VIP
Left panel, Competitive inhibition of [125I]VIP binding to CHO cell homogenates expressing hVPAC1 receptor by VIP and Bpa28-VIP. The data are expressed as percentage of initial specific binding in the absence of competitor. Right panel, Action of increasing concentrations of VIP and Bpa28-VIP on adenylyl cyclase activity in CHO cell homogenates. ○, Bpa28-VIP; •, VIP. Points are mean values from three studies; sem values are less than 4% of mean values.