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Photoaffinity Labeling of hVPAC1 Receptor and the D125M Receptor Mutant and Radiochemical Sequencing
A, CNBr-digestion products of covalent complex [125I-Bpa28]VIP/hVPAC1 receptor (lane 1) and [125I-Bpa28]VIP/D125M receptor mutant (lane 2) were resolved on SDS-NuPAGE gel. B, Radiochemical sequencing of digested peptides after photoaffinity labeling of the D125M hVPAC1 receptor mutant with the [125I-Bpa28]VIP probe. See Materials and Methods for details. MW, Molecular weight.
