Table 2.

Equilibrium parameters for CD-monitored trifluoroethanol (TFE) induced helix formation of CID variants determined in 20 mM sodium phosphate, pH 7.4, 150 mM NaCl, at 25°C.

DOI: http://dx.doi.org/10.7554/eLife.16059.018

CID variant	[TFE]50%* (%)	[TFE]50%† (%)	mD-N† (%−1)
1R‡	8.5 ± 1.3	7.6 ± 2.3	0.15 ± 0.02
2R	10.7 ± 0.9	12.0 ± 0.2	0.22 ± 0.01
Fish/tetrapod NCOA3	9.9 ± 0.7	10.0 ± 0.6	0.17 ± 0.01
Hsa NCOA1	-§	-§	0.15 ± 0.03
Hsa NCOA2	9.5 ± 1.7	-§	0.11 ± 0.02
Hsa NCOA3	5.6 ± 1.5	6.5 ± 0.9	0.18 ± 0.01

*The m_D-N value was shared among the datasets in the curve fitting; m_D-N = 0.17 ± 0.01 %⁻¹.

^†Free fitting of both [TFE]_50% and m_D-N.

^‡1R, the node around the time of the first whole genome duplication in the vertebrate lineage; 2R, the node around the time of the second whole genome duplication in the vertebrate lineage; Fish/tetrapod, the node where fish diverged from tetrapods; Hsa, Homo sapiens; Dre, Danio rerio (zebrafish); Pme, Petromyzon marinus, (sea lamprey); Dmel, Drosophila melanogaster (fruit fly).

^§Not well determined in the curve fitting.