Table 1.

Standard and real-time quantitative PCR primer sets.

Gene name	Primer sequence
Human TMEM176A
Standard PCR	Forward: 5′-ATGGGAACAGCCGACAGTGAT-3′	Reverse: 5′-CTAGATTCCACTCACTTCCAA-3′
Real-time QPCR	Forward: 5′-CATGGACATGCTGAAGGCCTTGTT-3′	Reverse: 5′-ACATTCTCCAGCAGTACAGCCACA-3′
Human TMEM176B
Standard PCR	Forward: 5′-ATGACGCAAAACACGGTGATT-3′	Reverse: 5′-TCACAGGACAATGGCAGTGGA-3′
Real-time QPCR	Forward: 5′-GCGAAGTCAAGAGAACCAATG-3′	Reverse: 5′-CTACTCCCAAGGAAACCAAGG-3′
Human GAPDH
Standard PCR	Forward: 5′-ATGGGGAAGGTGAAGGTCG-3′	Reverse: 5′-AGTGGTCGTTGAGGGCAAT-3′
Human 18S rRNA
Real-time QPCR	Forward: 5′-GCCCGAAGCGTTTACTTTG-3′	Reverse: 5′-CCCTCTTAATCATGGCCTCAG-3′