Table 2.
Normalized human TMEM176A and 176B protein expression levels from biopsied tissues of healthy normal and cancer patients. Human β-actin protein was used as normalization control. The protein levels were quantified and analyzed as described in the Materials and Methods section. Data are represented as means ± SEM. Statistical significance was calculated using Student’s t-test (p < 0.05, two-tailed distribution).
| Tissue samples | HsTMEM176A normalized protein levels (AU) | HsTMEM176B normalized protein levels (AU) | Sample size (n) |
|---|---|---|---|
| Breast cancer | 4.53 ± 0.48 | 4.64 ± 0.52 | 9 |
| Breast normal | 4.65 ± 1.66 | 2.93 ± 1.57 | 4 |
| Lung cancer | 3.60 ± 0.43* | 3.26 ± 0.56 | 10 |
| Lung normal | 1.71 ± 0.52 | 2.14 ± 0.88 | 5 |
| Liver cancer | 2.30 ± 0.54 | 3.23 ± 1.00 | 9 |
| Liver normal | 1.25 ± 0.12 | 1.11 ± 0.07 | 5 |
| Stomach cancer | 4.58 ± 0.49 | 4.12 ± 0.35 | 10 |
| Stomach normal | 4.14 ± 0.63 | 3.62 ± 0.59 | 3 |
| Colon cancer | 4.51 ± 0.50 | 3.96 ± 0.55 | 10 |
| Colon normal | 3.48 ± 0.97 | 3.06 ± 0.83 | 5 |
| Kidney cancer | 2.17 ± 0.43 | 4.00 ± 0.90 | 9 |
| Kidney normal | 1.89 ± 0.34 | 2.70 ± 0.42 | 5 |
| Skin cancer (melanoma) | 2.84 ± 0.57# | 4.14 ± 1.12 | 9 |
| Skin normal | 5.26 ± 1.63 | 3.42 ± 1.11 | 3 |
| Lymphoid cancer (lymphoma) | 5.14 ± 0.53* | 4.58 ± 0.46** | 10 |
| Lymphoid normal | 2.89 ± 0.64 | 1.68 ± 0.33 | 5 |
| Ovary cancer | 4.18 ± 0.51 | 3.11 ± 0.42 | 10 |
| Ovary normal | 3.05 ± 0.69 | 3.20 ± 0.98 | 4 |
| Prostate cancer | 5.03 ± 0.36 | 5.45 ± 0.27# | 8 |
| Prostate normal | 4.66 ± 0.79 | 4.05 ± 0.82 | 5 |
| Endometrial cancer | 4.15 ± 0.42 | 3.26 ± 0.50 | 10 |
| Endometrium normal | 4.02 ± 0.80 | 3.63 ± 0.64 | 5 |
*
p < 0.05.
**
p < 0.001.
#
p = 0.08 (barely significant value).