REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Chicken polyclonal anti-GFP	Abcam	Cat# ab13970; RRID:AB_300798
Rabbit polyclonal anti-β-galactosidase	MP Biomedicals	Cat# 0855978; RRID:AB_2334737
Mouse monoclonal anti-rat CD2	Bio-Rad (Formerly AbD Serotec)	Cat# MCA154GA; RRID: AB_566608
Mouse monoclonal anti-Prospero	DSHB	Cat# MR1A; RRID: AB_528440
Rabbit polyclonal anti-RFP	MBL International	Cat# PM005; RRID:AB_591279
Mouse monoclonal anti-lamin DmO	DSHB	Cat# ADL84.12; RRID: AB_528338
Rat monoclonal anti-alpha-tubulin	Bio-Rad (Formerly AbD Serotec)	Cat# MCA78G; RRID: AB_325005
Rat monoclonal anti-cadherin, DE- (extracellular domain)	DSHB	Cat# DCAD2; RRID: AB_528120
Mouse monoclonal anti-Delta, extracellular domain	DSHB	Cat# C594.9B; RRID:AB_528194
Rabbit polyclonal anti-phospho-Histone H3 (Ser10)	Millipore	Cat# 06-570; RRID: AB_310177
Rabbit polyclonal anti-GFP	Torrey Pines Biolabs	Cat# TP401 071519; RRID: AB_10013661
Goat anti-chicken IgY (H+L) secondary antibody, Alexa Fluor® 488	Abcam	Cat# ab7114; RRID: AB_955348
Goat anti-chicken IgY (H+L) secondary antibody, Alexa Fluor® 488	ThermoFisher Scientific	Cat# A11039; RRID: AB_2534096
Donkey anti-rabbit IgG (H+L) secondary antibody, Alexa Fluor® 488	ThermoFisher Scientific	Cat# A21206; RRID:AB_2535792
Donkey anti-rabbit IgG (H+L) secondary antibody, Alexa Fluor® 546	ThermoFisher Scientific	Cat# A10040; RRID: AB_2534016
Goat anti-rabbit IgG (H+L) secondary antibody, Alexa Fluor® 633	ThermoFisher Scientific	Cat# A21070; RRID: AB_2535731
Donkey anti-mouse IgG (H+L) secondary antibody, Alexa Fluor® 555	ThermoFisher Scientific	Cat# A31570; RRID: AB_2536180
Goat anti-mouse IgG (H+L) secondary antibody, Alexa Fluor® 633	ThermoFisher Scientific	Cat# A21050; RRID: AB_2535718
Goat anti-mouse IgG2a secondary antibody, Alexa Fluor® 555	ThermoFisher Scientific	Cat# A21137; RRID: AB_2535776
Goat anti-mouse IgG1 secondary antibody, Alexa Fluor® 488	ThermoFisher Scientific	Cat# A21121; RRID: AB_2535764
Goat anti-mouse IgG1 secondary antibody, Alexa Fluor® 647	ThermoFisher Scientific	Cat# A21240; RRID: AB_2535809
Goat anti-rat IgG (H+L) secondary antibody, Alexa Fluor® 633	ThermoFisher Scientific	Cat# A21094; RRID: AB_2535749
Bacterial and Virus Strains
Biological Samples
Chemicals, Peptides, and Recombinant Proteins
Demecolcine ≥98 % (HPLC)	Sigma	Cat# D7385; CAS: 477-30-5
Bleomycin (sulfate) ≥95%	Cayman Chemical Company	Cat# 13877; CAS: 9041-93-4
Sucrose, ultra-pure grade	LabScientific	Cat# 0335; CAS: 57-50-1
Deep blue food coloring	Esco Foods	Cat# 34-400
Pierce™ 16% Formaldehyde (w/v), methanol-free	ThermoFisher Scientific	Cat# 28908; CAS: 50-00-0
Molecular Probes™ Rhodamine Phalloidin	Invitrogen	Cat# R415
4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI)	Sigma	Cat# D9542; CAS: 28718-90-3
Critical Commercial Assays
Deposited Data
Experimental Models: Cell Lines
Experimental Models: Organisms/Strains
D. melanogaster: NRE-lacZ; ;TM2/TM6B, Hu, Tb	Sarah Bray; Furriols et al., 2001	N/A
D. melanogaster: ; esg-GAL4, UAS-GFP;	Craig Micchelli; Micchelli et al., 2006	N/A
D. melanogaster: NRE-lacZ; esg-GAL4, UAS-GFP/CyO;	This paper	N/A
D. melanogaster: w[*]; P{w[+mC]=UAS-CD2}5;	Bloomington Drosophila Stock Center	BDSC: 1284; FlyBase: FBst0001284
D. melanogaster: ; vkg-GFP/CyO;	Jin Jiang; Tian et al., 2014	N/A
D. melanogaster: NRE-lacZ; esg-GAL4, UAS-CD2, vkg-GFP/CyO;	This paper	N/A
D. melanogaster: y, w, hsFLP, tub-GAL4, UAS-nuc-GFP; ; FRT82B tubGAL80	Gary Struhl	N/A
D. melanogaster: ; ; P{ry[+t7.2]=neoFRT}82B ry[506]	Bloomington Drosophila Stock Center	BDSC: 2035; FlyBase: FBst0002035
D. melanogaster: NRE-lacZ; ; FRT82B ry[506]/TM2	This paper	N/A
D. melanogaster: wcall net [1118]; ; P{w[+mC]=His2Av-mRFP1}III.1 P{w[+mW.hs]=FRT(w[hs])}2A	Bloomington Drosophila Stock Center	BDSC: 34498; FlyBase: FBst0034498
D. melanogaster: y, w, hsFLP; ; FRT2A, His-GFP	Zheng Guo; Guo et al., 2009	N/A
D. melanogaster: NRE-lacZ; esg-GAL4, UAS-CD2/CyO;	This paper	N/A
D. melanogaster: UAS-anillin-GFP; Sp/CyO; P/TM3	Don Fox	N/A
D. melanogaster: ; Myo1A-GAL4, UAS-GFP/CyO;	Bruce Edgar; Jiang et al., 2009	N/A
D. melanogaster: ; esg-GFP[P01986];	Alan Spradling; Buszczak et al., 2007
D. melanogaster: w[*]; ; P{y[+t7.7] w[+mC]=10×UAS-IVS-mCD8∷RFP}attP2	Bloomington Drosophila Stock Center	BDSC: 32218; FlyBase: FBst0032218
D. melanogaster: y, w[122]; esg-GFP/CyO; 10× UAS-IVS-mCD8∷RFP/TM6B, Hu, Tb	This paper	N/A
D. melanogaster: P{w[+mC] GAL4∷Hsp70B [mex.P5′2.1]=mexGAL4}	Philips et al., 2006	N/A
D. melanogaster: ; ; P{GMR28E03-GAL4}attP2	Bloomington Drosophila Stock Center	BDSC: 45546; FlyBase: FBsf0000163290
D. melanogaster: y[1] w[*]; Pin[Yt]/CyO; P{w[+mC]=UAS-mCD8∷GFP.L}LL6	Bloomington Drosophila Stock Center	BDSC: 5130; FlyBase: FBst0005130
D. melanogaster: NRE-lacZ; ; 28E03-GAL4, UAS-mCD8∷GFP	This paper	N/A
D. melanogaster: ; pBID-UASC-FLP attP16/SM6a;	This paper	N/A
D. melanogaster: w; pBID-UASC-FLP/CyO; 28E03-GAL4/TM6B, Hu, Tb	This paper	N/A
D. melanogaster: ; Act5C FRT draf+ FRT lacZ/CyO; TM2/TM6B, Hu, Tb	Laura Johnston	N/A
D. melanogaster: w[1118]; P{w[+m*]=NRE-EGFP.S}1	Bloomington Drosophila Stock Center	BDSC: 30728; FlyBase: FBst0030728
D. melanogaster: w; Act5C FRT draf+ FRT lacZ/CyO; NRE-EGFP/TM2	This paper	N/A
D. melanogaster: w; Act5C FRT draf+ FRT lacZ/CyO; 28E03-GAL4/TM6B, Hu, Tb	This paper	N/A
D. melanogaster: w; esg-GFP/CyO; pBID-UASCFLP/TM6B, Hu, Tb	This paper	N/A
D. melanogaster: w[*]; ; TM6B, P{w[+mC]=tubPGAL80} OV3, Tb[1]/TM3, Sb[1]	Bloomington Drosophila Stock Center	BDSC: 9490; FlyBase: FBst0009490
D. melanogaster: tub-GAL4, UAS-dsRed/FM7; ; MKRS/TM6B, tubP-GAL80, Tb	This paper	N/A
D. melanogaster: Oregon R.	Bloomington Drosophila Stock Center	BDSC: 2376; FlyBase: FBst0002376
D. melanogaster: ; kuz[e29-4]/CyO;	Bloomington Drosophila Stock Center	BDSC: 5804; FlyBase: FBst0005804
D. melanogaster: ; ln2LR, Gla, Bc/CyO, WeeP-GFP;	Brian McCabe	N/A
D. melanogaster: y w; kuz[e29-4]/CyO, WeeP-GFP	This paper	N/A
Oligonucleotides
Primer for cloning of flippase (FLP), forward: AACTTAAAAAAAAAAATCAAAATGCCGCAGTTTGATATCC	eurofins, Fisher Scientific	www.operon.com/fihersci
Primer for cloning of flippase (FLP), reverse: CTATTAAATACGGCGATTGATGTAGGAGCTC	eurofins, Fisher Scientific	www.operon.com/fishersci
Recombinant DNA
Plasmid: pBID-UASC-G	Wang et al., 2012	Addgene plasmid #35202
Software and Algorithms
Fiji	Schindelin et al., 2012	https://fiji.sc/
MetaMorph	Molecular Devices	https://www.moleculardevices.com/
Igor Pro	WaveMetrics	https://www.wavemetrics.com/
Prism 6	GraphPad	http://www.graphpad.com/scientific-software/prism/
Other
BuzzPlugs™ vial closures	Fisher Scientific	Cat# AS273
Whatman™ 3MM chromatography paper	Fisher Scientific	Cat# 05-714-5