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. Author manuscript; available in PMC: 2017 Oct 26.
Published in final edited form as: Nature. 2017 Apr 26;545(7652):108–111. doi: 10.1038/nature22078

Figure 3. Ataxin-3 polyQ domain contributes to ataxin-3 interaction with beclin 1.

Figure 3

a,b,c, Constructs were transfected in HeLa cells for 24 h. a, Cell lysates were immunoprecipitated with anti-GFP antibody and immunocomplexes analysed with anti-FLAG antibody. b,c, Cell lysates were immunoprecipitated with anti-FLAG antibody and immunocomplexes analysed with anti-GFP antibody. Gel source data in Supplementary Fig. 1. d, Structural docking modelling reveals two interactions sites between beclin 1 ECD and PolyQ7. Surface charge illustration of human beclin 1 ECD showing high scored docking pose of polyQ7 stretch (docking scores for site 1 and site 2 are -10.394 and -10.721, respectively). Full structural analysis in Extended Data Fig. 5.