Table 2.
Genes regulated by PEMF during hBMSCs proliferation by microarray analysis. Cells were from a normal 27-year-old female. Total RNA was isolated at day 5 after 2 h of PEMF treatment and used for microarray assays as described in Materials and Methods. Analysis by Student's t-test.
| Gene symbol | Gene title | Fold-change (Avg PEMF versus avg controls) |
p |
|---|---|---|---|
| Cell adhesion and binding and cytoskeletal and structural proteins | |||
| MMP1 | Matrix metallopeptidase 1 (interstitial collagenase) | 4.57 | 2.16E − 03 |
| PRC1 | Protein regulator of cytokinesis 1 | 3.22 | 4.34E − 04 |
| CCBE1 | Collagen and calcium binding EGF domains 1 | 2.97 | 1.32E − 04 |
| CLDN1 | Claudin 1 | 2.71 | 2.26E − 04 |
| CENPK | Centromere protein K | 2.40 | 5.36E − 03 |
| GAS2L3 | Growth arrest-specific 2 like 3 | 2.39 | 4.74E − 03 |
| CLDN11 | Claudin 11 | 2.31 | 5.59E − 04 |
| NUSAP1 | Nucleolar and spindle associated protein 1 | 2.16 | 5.04E − 03 |
| COL15A1 | Collagen, type XV, alpha 1 | 2.12 | 1.64E − 04 |
| HAPLN1 | Hyaluronan and proteoglycan link protein 1 | 2.03 | 1.37E − 04 |
| IBSP | Integrin-binding sialoprotein | 1.97 | 2.17E − 03 |
| FBN2 | Fibrillin 2 | −1.12 | 1.98E − 02 |
| COL14A1 | Collagen, type XIV, alpha 1 | −2.30 | 1.56E − 02 |
| MMP12 | Matrix metallopeptidase 12 (macrophage elastase) | −3.27 | 1.19E − 04 |
| MGP | Matrix Gla protein | −3.98 | 2.80E − 06 |
|
| |||
| p53 signaling pathway, apoptosis, and survival antiapoptotic TNFs/NF-kB/IAP pathway | |||
| BIRC5 | Baculoviral IAP repeat containing 5 | 3.30 | 3.60E − 06 |
| GTSE1 | G-2 and S-phase expressed 1 | 2.48 | 1.02E − 03 |
| SESN3 | sestrin 3 | −2.47 | 3.72E − 07 |
|
| |||
| Cell cycle role of APC (anaphase-promoting complex) in cell cycle regulation, cell cycle/checkpoint control | |||
| CDK1 | Cyclin-dependent kinase 1 | 5.07 | 1.01E − 03 |
| CDC20 | Cell division cycle 20 homolog (S. cerevisiae) | 3.00 | 3.32E − 04 |
| CCNB2 | Cyclin B2 | 2.41 | 1.83E − 03 |
| NDC80 | NDC80 kinetochore complex component homolog (S. cerevisiae) | 2.38 | 7.59E − 04 |
| TYMS | Thymidylate synthetase | 2.36 | 9.43E − 05 |
| CCNB1 | Cyclin B1 | 2.35 | 2.17E − 03 |
| NEK2 | NIMA- (never in mitosis gene a-) related kinase 2 | 2.12 | 2.19E − 02 |
| CCNA2 | Cyclin A2 | 1.95 | 6.42E − 03 |
| TTK | TTK protein kinase | 1.95 | 1.77E − 02 |
|
| |||
| Akt signaling | |||
| CCL2 | Chemokine (C-C motif) ligand 2 | 2.83 | 6.76E − 05 |
| CSF2RB | Colony stimulating factor 2 receptor, beta, low-affinity | 2.31 | 2.04E − 03 |
|
| |||
| Other receptor, kinase, and regulator | |||
| CDKN3 | Cyclin-dependent kinase inhibitor 3 | 2.45 | 2.32E − 04 |
| PDGFRA | Platelet-derived growth factor receptor, alpha polypeptide | 2.26 | 2.52E − 05 |
| CTSC | Cathepsin C | 2.14 | 5.32E − 04 |
| LEPR | Leptin receptor | −2.19 | 1.46E − 04 |
| FGFR2 | Fibroblast growth factor receptor 2 | −2.04 | 8.83E − 05 |