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. 2017 Apr 23;2017:2960502. doi: 10.1155/2017/2960502

Figure 2.

Figure 2

PCR-RFLP assay for PECAM-1 gene (+1688 A/G) and (+373 C/G) polymorphism. Representative electrophoresis of PCR products cleaved with restriction endonuclease Pvu II and Nhe I was shown. Lane M: DNA molecular weight marker. (a) PECAM-1 (+373 C/G) gene. Lane 1: 373CC homozygous genotype, 188 bp; Lane 2: 373CG heterozygous genotype, 255 bp and 188 bp; Lane 3: 373GG homozygous genotype, 255 bp. The 37 bp in 373 CC and 373 CG genotypes was not visible in electrophoresis gel due to its too small size. (b) PECAM-1 (+1688 A/G) gene. Lane 1: 1688AG heterozygous genotype, 338 bp, 219 bp, and 119 bp; Lane 2: 168AA homozygous genotype, 338 bp; and Lane 3: 1688GG homozygous genotype, 219 bp and 119 bp. PECAM-1: platelet endothelial cell adhesion molecule-1.