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. 2017 Mar 16;27(6):555–567. doi: 10.1093/glycob/cwx019

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© The Author 2017. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Fig. 4. — ESI-IT-MS analysis of cellular nucleotide sugar pools from T. forsythia strains. (A) CMP-activated Pse5Am7Gra (m/z 683.3) was detected in the T. forsythia ATCC 43037 wild-type and in the ΔTanf_01245 mutant, whereas this mass was absent in a Pse biosynthesis deficient strain (ΔpseC) which served as a negative control. (B) In T. forsythia UB4 wild-type and in the ΔTFUB4_00887 mutant, a m/z 654.3 peak was identified, which was attributed to a CMP-activated Leg derivative (CMP-Leg*). This mass is consistent with having Ac and Gc modifications on Leg, based on calculation. Notably, this peak was absent in the Legbiosynthesis deficient strain (ΔlegC) which served as a negative control. Relative peak intensities are given on the y axis.