. 2017 Mar 16;27(6):555–567. doi: 10.1093/glycob/cwx019

Table II.

Oligonucleotide primers used for PCR amplification reactions

Primers	Sequence (5′–3′)
1^a	GGTACCCCCGATAGCTTCCGCTATTGC
2	CTACGAAGGATGAAATTTTTCAGGG
3^a	GCAATAGCGGAAGCTATCGGGGGTACC
4	CCCTGAAAAATTTCATCCTTCGTAG
48	GTCAGATAGGCCTAATGACTGGC
76	TTATAAAAGCCAGTCATTAGGCCTATCTGAC
77^b	aatcaGCATGC GGTACCTTATAAAAGCCAGTCATTAGGCCTATCTGAC
118	ATGGCTACAATGGTCTGTAATTATCTTC
119	TTATATTACTGTTATTGTTCGTAGATCC
120	CCATGATAATCTCGACTTCGG
121	GCAATAGCGGAAGCTATCGGGGGTACCATTCCTATCTCTTGAAGGATAGG
122	CCCTGAAAAATTTCATCCTTCGTAGGTATAGAGGTACAATGGATATAGGGC
123	GCACCCATTTATCTAAATAATCTTC
124	GGCCCTCAACCTTTTCTGGC
125	CCTATCCTTTAGGTATCTATATG
126^b	CGAACAATAACAGTAATATAATGTACAATGAACTTTAATAAAATTGATTTAGAC
127	aatcaCATATGGGTACCTTATAAAAGCCAGTCATTAGGCCTATCTGAC
128	aatcaGGTACCGTATAGAGGTACAATGGATATAGGGC
130	CCAATTGTCTAAATCAATTTTATTAAAGTTCATTGTACATTATATTACTGTTATTGTTCGTAGATCC
133	aatcaTGTACATTATATTACTGTTATTGTTCGTAGATCCTC
134	aatcaCTCGAGCCATGATAATCTCGACTTCGG
140	aatcaCATATGGCACCCATTTATCTAAATAATCTTC
152	CTAAATTTCTTTCATAATAATTCTTTGTATAATGATTGATGTTTTAAATTTAGAATGGACC
153	GGTCCATTCTAAATTTAAAACATCAATCATTATACAAAGAATTATTATGAAAGAAATTTAG
460^c	ATGACAAAAAAGAAATTGCCCGTTCGTTTTAC
461^c	CTACGAAGGATGAAATTTTTCAGGGACAAC
474^d	CGGGCAATTTCTTTTTTGTCATTTCTTAATGTAATCTAAGTCCAACCG
475^d	TTGTAGCAGAACTATCAGCCAATCAC
476^d	GTTGTCCCTGAAAAATTTCATCCTTCGTAGGTATAGAGGTACAATGGATATAG
477^d	CCCAGACTCTTCTTTAACAAGAAACC
512	TGCAGGCTGCAATTGATTCC
513	GATCCCACGTGAAAGCAAATA
524	GTAAAACGAACGGGCAATTTCTTTTTTGTCAT
525	CCCTGAAAAATTTCATCCTTCGTAG
530	CGTATGATATTTGCAGTCTTG
531	GTAATAACCATATCTGCCTCTGGAAC
540	CAACAATTGTAGCAGAACTATCAGCC
541	aatcaGGTACCTATAAGTATAGAGGTACAATGGATATAGG
542	aatcaGCATGCGGTATCTATATGAAGTACGCACC
543	ctgaCTCGAGCAACAATTGTAGCAGAACTATC
565	CTAAATCAATTTTATTAAAGTTCAT
575	TCTAAATTAAGAATATCCATTTCTTAATGTAATCTAAGTCCAACCGCATTCC
576	GACTTAGATTACATTAAGAAATGGATATTCTTAATTTAGAATGGACTTCC

Nucleotides used for overlap-extension PCRs are written in bold, artificial restriction sites are underscored. Lowercase letters indicate artificially introduced bases to improve restriction enzyme digestion.

^aFriedrich et al. (2017).

^bZarschler et al. (2009).

^cTomek et al. (2014).

^dSequence (3′–5′).