Table 2.
Effects of chronic saline (Sal) or nicotine (14 or 40 mg/kg/day) administration and withdrawal on secondary performance measures for wildtype (WT) and α7 nAChR knockout (KO) mice in the 5-choice continuous performance test.
Effects of chronic nicotine (“Nic” at 14 or 40 mg/kg/day vs. saline, “Sal”) administration and withdrawal on secondary performance measures for WT and α7 nAChR KO mice in the 5C-CPT. There was no main effect of genotype or nicotine on total trials, % omissions, or % premature responses. There was a significant nicotine × genotype interaction on % omissions 33 days after pump implantation since the low dose of nicotine resulted in fewer omitted trials compared to saline and the high nicotine dose for the WT mice but not KO mice.
| Pre-Treatment | Nicotine (Day 33) | Withdrawal (4 h) | ||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
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| Mean ± SEM | Genotype | Treatment Group | Genotype × Group | Mean ± SEM | Genotype | Nicotine | Genotype × Nicotine | Mean ± SEM | Genotype | Nicotine | Genotype × Nicotine | |||||||||||
| F(1, 39) | p | F(2,39) | p | F(2,39) | p | F(1, 39) | p | F(2,39) | p | F(2,39) | p | F(1, 39) | p | F(2,39) | p | F(2,39) | p | |||||
| Total Trials | WT - Sal | 113.07±3.44 | <1 | ns | <1 | ns | 2.2 | ns | 243.20±2.01 | <2 | ns | <1 | ns | <2 | ns | 237.80±6.99 | <1 | ns | <1 | ns | <2 | ns |
| WT - Nic (14) | 116.76±1.71 | 245.00±0.00 | 220.00±17.86 | |||||||||||||||||||
| WT - Nic (40) | 116.95±1.69 | 227.00±12.54 | 207.29±19.48 | |||||||||||||||||||
| KO - Sal | 117.70±1.23 | 227.56±11.68 | 219.00±9.29 | |||||||||||||||||||
| KO - Nic (14) | 108.50±5.93 | 224.50±12.64 | 222.13±21.97 | |||||||||||||||||||
| KO - Nic (40) | 113.74±2.43 | 236.56±4.72 | 239.00±5.60 | |||||||||||||||||||
| % Omissions | WT - Sal | 22.03±4.59 | <1 | ns | <1 | ns | <2 | ns | 26.22±6.67 | <1 | ns | <1 | ns | 4.1 | 0.02 | 28.06±8.09 | <1 | ns | <1 | ns | <2 | ns |
| WT - Nic (14) | 15.25±1.38 | 15.66±1.73 | 31.43±6.07 | |||||||||||||||||||
| WT - Nic (40) | 20.41±3.11 | 33.51±4.26 | 37.03±4.01 | |||||||||||||||||||
| KO - Sal | 16.43±1.79 | 30.50±7.38 | 37.84±8.53 | |||||||||||||||||||
| KO - Nic (14) | 21.04±4.69 | 36.44±6.31 | 32.66±10.73 | |||||||||||||||||||
| KO - Nic (40) | 18.04±4.18 | 21.56±6.61 | 23.39±6.01 | |||||||||||||||||||
| % Premature Responses | WT - Sal | 3.79±0.81 | <1 | ns | <1 | ns | <1 | ns | 4.13±1.13 | <1 | ns | <1 | ns | <1 | ns | 3.96±1.71 | 2.0 | ns | <1 | ns | <1 | ns |
| WT - Nic (14) | 8.18±3.66 | 3.50±1.01 | 4.47±1.83 | |||||||||||||||||||
| WT - Nic (40) | 5.37±1.92 | 4.79±2.99 | 6.03±3.35 | |||||||||||||||||||
| KO - Sal | 5.79±2.03 | 4.73±1.85 | 8.04±3.19 | |||||||||||||||||||
| KO - Nic (14) | 6.18±2.02 | 4.50±1.98 | 6.45±2.24 | |||||||||||||||||||
| KO - Nic (40) | 6.25±2.94 | 5.29±1.97 | 12.31±5.66 | |||||||||||||||||||