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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Gut. 2016 Nov 7;67(3):473–484. doi: 10.1136/gutjnl-2016-312794

Figure 4. Western blotting confirmation of increased levels of BAG4, IL6ST, VWF, EGFR, and CD44.

Figure 4

BAG4, IL6ST, VWF, EGFR, and CD44 levels were examined in plasma from people with villous adenoma, carcinoma-in-situ, or invasive cancer cases compared to controls (collected prior to colonoscopy). Albumin and IgG depleted plasma proteins (30 µg) were separated on a denaturing 4–12% Bis-Tris gel under reducing conditions. After immunoblot and primary antibody incubation, specific bands were visualized with a fluorescently-labeled secondary antibody. Statistical significance: *, p<0.05; **, p<0.01; ***, p<0.001; ****, p<0.0001.