Immunohistochemistry staining of BAG4, IL6ST and CD44 was scored based on Allred’s method and statistical significance was calculated between normal colon tissue and each case category. Tested tissue samples included 217 normal subject colon tissues, 263 adenomas, 57 stage I, 167 stage II, 128 stage III and 84 stage IV cancer cases. Statistical significance: *, p<0.05; ***, p<0.001; ****, p<0.0001.