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Caspase 3 activity assay. The assay was performed using commercially available kit (Abcam ab39401) and the assay samples were assessed at 405 nm. The lower caspase-3 activity indicated small level of apoptotic activity in the samples. Caspase-3 activity was significantly lower in samples from day 3 treatment group of animals as compared to the sham operated controls (*P<0.05).
