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. 2017 May 8;11:131. doi: 10.3389/fncel.2017.00131

Figure 1.

Figure 1

Human induced pluripotent stem cells (iPSCs)-derived serotonergic neurons are sensitive to 5,7-DHT in vitro. (A) Phase contrast images and immunostaining images of the cells treated with different concentrations of 5,7-DHT. (B) Quantification of Serotonin positive or TH positive cells in (A) (n = 3). Data are represented as mean ± SEM. (C) Phase contrast images and immunostaining images of the cells treated with different concentrations of 6-OHDA. (D) Quantification of Serotonin positive or TH positive cells in (C) (n = 3). Data are represented as mean ± SEM. *P < 0.05, Student’s t-test. Scale bar: 50 μm; Red: TH+ cells; Green: Serotonin+ cells; Blue: Hoechst staining (Ho).