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. 1976 Apr;57(4):651–655. doi: 10.1104/pp.57.4.651

Synergetic Cultures of Glycine max Root Cells and Rhizobia Separated by Membrane Filters 1

Minocher Reporter a
PMCID: PMC542091  PMID: 16659545

Abstract

When suspension cultures of actively growing soybean (Glycine max L.) root cells were separated by two or three membrane filters from suspension cultures of the bacteria, a synergetic (cooperative) activation of nitrogenase was observed in the Rhizobium japonicum used in the bacterial side. Either plant cells or plant cell-conditioned medium was needed for this activation to take place. Both acetylene reduction and hydrogen evolution by the activated R. japonicum persisted for several days after removal from the apparatus when (a) a suitable carbon source was provided, (b) oxygen supply was limited, and (c) growth of bacteria was suppressed by lowering of ammonia and nitrate concentrations. Activation could also take place when the bacteria were placed in media to which plant cell-conditioned medium was added. The advantages of this method for studies on symbiosis are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Child J. J., Larue T. A. A simple technique for the establishment of nitrogenase in soybean callus culture. Plant Physiol. 1974 Jan;53(1):88–90. doi: 10.1104/pp.53.1.88. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Constabel F., Kurz W. G., Chatson B., Gamborg O. L. Induction of partial synchrony in soybean cell cultures. Exp Cell Res. 1974 Mar 30;85(1):105–110. doi: 10.1016/0014-4827(74)90218-3. [DOI] [PubMed] [Google Scholar]
  3. Gamborg O. L., Miller R. A., Ojima K. Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res. 1968 Apr;50(1):151–158. doi: 10.1016/0014-4827(68)90403-5. [DOI] [PubMed] [Google Scholar]
  4. Holsten R. D., Burns R. C., Hardy R. W., Hebert R. R. Establishment of symbiosis between Rhizobium and plant cells in vitro. Nature. 1971 Jul 16;232(5307):173–176. doi: 10.1038/232173a0. [DOI] [PubMed] [Google Scholar]
  5. Keister D. L. Acetylene reduction by pure cultures of Rhizobia. J Bacteriol. 1975 Sep;123(3):1265–1268. doi: 10.1128/jb.123.3.1265-1268.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. Phillips D. A. Factors affecting the reduction of acetylene by Rhizobium-soybean cell associations in vitro. Plant Physiol. 1974 Jan;53(1):67–72. doi: 10.1104/pp.53.1.67. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Phillips D. A., Torrey J. G. Studies on cytokinin production by Rhizobium. Plant Physiol. 1972 Jan;49(1):11–15. doi: 10.1104/pp.49.1.11. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Reporter M., Hermina N. Acetylene reduction by transfilter suspension cultures of Rhizobium japonicum. Biochem Biophys Res Commun. 1975 Jun 16;64(4):1126–1133. doi: 10.1016/0006-291x(75)90811-6. [DOI] [PubMed] [Google Scholar]
  10. Streeter J. G., Bosler M. E. Comparison of in vitro and in vivo assays for nitrate reductase in soybean leaves. Plant Physiol. 1972 Mar;49(3):448–450. doi: 10.1104/pp.49.3.448. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. VALERA C. L., ALEXANDER M. NODULATION FACTOR FOR RHIZOBIUM-LEGUME SYMBIOSIS. J Bacteriol. 1965 Apr;89:1134–1139. doi: 10.1128/jb.89.4.1134-1139.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]

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