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. 2017 May 8;8:752. doi: 10.3389/fmicb.2017.00752

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Copyright © 2017 Moitinho-Silva, Steinert, Nielsen, Hardoim, Wu, McCormack, López-Legentil, Marchant, Webster, Thomas and Hentschel.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Alpha diversity of HMA and LMA sponge samples. Richness (A–C) and diversity (D–F) metrics were calculated for each sample (n = 575) using rarefied OTU abundances. Estimated mean and 95% confidence intervals were obtained from linear mixed models of alpha diversity metrics. The effect of HMA-LMA status was tested with Likelihood ratio tests. In this procedure, two linear models with mixed effects were compared, the full model and the null model. All metrics were significantly greater (ANOVA, P ≤ 0.001) in the HMA than in the LMA group.