FIGURE 1.

Mouse in vivo bioluminescence imaging after bilateral i.m. injection with SAM encoding firefly luciferase with or without RSV F. (A) Dorsal bioluminescence view of mice immunized with SAM expressing Luciferase (luc) or SAM coexpressing luciferase and RSV F (luc/F). (B) Plot of the average radiance calculated from the bioluminescence images. Each dot represents the mean of the dorsal photon count of 10 posterior legs per group of mice, expressed as photons per second per square centimeter per steradian (p/s/cm²/sr). The error bars represent the SEM. The bioluminescence intensity was evaluated at days 1, 3, 7, 14, 21, 28, 35, and 49 postimmunization. The limit of detection is 1 × 10³ p/s/cm²/sr. *p < 0.05, **p < 0.01. (C) Schematic representation of the polycistronic luc/F RNA. (D) Immunoblot analysis of RSV F in BHK cell extracts transfected with the polycistronic RNA.