Table 2.
The effect of controlling the dissolved oxygen tension (pO2) on biomass, lipid and citric acid production by Y. lipolytica grown in nitrogen limited medium with glucose, glycerol or blend of both
| Substrate | µ (h−1) | Biomass | Lipid | Citric | Citric acid yield in g/g substrate | |
|---|---|---|---|---|---|---|
| Log-phase | (g/L) | (g/L) | (g/L) | (Maximum) in stationary phase | overall | |
| pO2 uncontrolled | ||||||
| Glucose | 0.13 | 12.8 | 3 | 16.1 | 0.44 | 0.23 |
| Glycerol | 0.15 | 9.03 | 3.6 | 23.4 | 0.39 | 0.39 |
| Blend | 0.16 | 12.3 | 4.25 | Varied | 0.35 | 0.29 |
| Blend with BH | 0.11 | 14.2 | 2.4 | Varied | 0.41 | 0.27 |
| pO2 controlled | ||||||
| Glucose | 0.2 | 12.2 | 2.4 | 55.0 | 0.83 | 0.58 |
| Glycerol | 0.21 | 11.03 | 2.1 | 17.8 | 0.3 | 0.27 |
| Blend | 0.22 | 11.9 | 2.4 | 50.1 | 0.76 | 0.6 |
| Blend with BH | 0.18 | 16.7 | 2.5 | 53.5 | 0.8 | 0.6 |