Skip to main content
. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Mol Genet Metab. 2017 Mar 1;120(4):317–324. doi: 10.1016/j.ymgme.2017.02.009

Figure 3.

Figure 3

Isothermal titration calorimetry (ITC) binding assays of L-Trp show two binding sites with distinct affinities to the wild-type TDO (left panel) and Met108Ile variant (right panel). The experiments were performed under O2-free conditions in an anaerobic chamber.