Fig. 2.

Identification of candidate chitin-HA oligos in fractionated lyase digests of SeHAS HA. HA was synthesized, purified, treated with HA lyase and the digest was then fractionated by solid phase extraction over Sep-Pak C-18 cartridges as described in Methods. The starting digest (A) and the Wash 1 (B), Wash 2 (C), Wash 3 (D) and ACN (E) fractions were examined by MALDI-TOF MS in negative mode. Labels indicate m/z peaks corresponding to M⁻¹ ions for many of the candidate dH_n and G_nH_n species listed in Tables I and II. Panel E is at a different scale than panels (A–D). Note that signals for some minor species such as G₅H_2,3 (at m/z 1790 and 2169) are not easily discerned at the scale used to show the more abundant species.