Fig. 2.

Capture of mRNA-binding proteins. A) Schematic of mRNA interactome capture. B) Silver-stained SDS-PAGE of yeast cell lysate (Input) and eluates after interactome capture from 4tU-labeled cells. C) Western blot analysis of mRNA interactome capture; cell lysate (Input) and eluates from capture experiments (PAB1, polyA binding protein; PUB1, polyU binding protein 1; H4, histone 4). D) Western blotting of series of UV crosslinking ranging from 0 to 7.2 J/cm².