Table III.

Reaction of PpBADH with substituted benzaldehydes^a

Substrate	Km (µM)	kcat (s−1)	kcat/Km (M−1 s−1)
Benzaldehyde	4.4 ± 2.2	156 ± 29	3.5 × 107
Benzaldehyde-α-d	3.9 ± 0.5	192 ± 11	4.9 × 107
p-methoxybenzaldehyde	2.0 ± 0.3	116 ± 4	5.8 × 107
p-chlorobenzaldehyde	1.2 ± 0.2	135 ± 2	11 × 107
p-nitrobenzaldehyde	9.3 ± 0.8	95 ± 3	1.0 × 107

^aReactions were carried out in TAPS buffer (0.1 M, pH 8.5) containing 0.1 M KCl, 1 mM NAD⁺ and 1 mM DTT at 30°C. Data were treated as described in Materials and methods.